Interleukin-10 (IL10), Macaca mulatta, recombinant

Organism: Macaca mulatta (Rhesus macaque). Source: Mammalian cell. Expression Region: 19-178aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal DEEP1-tagged and C-terminal 6xHis-tagged. Target Protein Sequence: SPGQGTQSEN SCTRFPGNLP HMLRDLRDAF SRVKTFFQMK DQLDNILLKE SLLEDFKGYL GCQALSEMIQ FYLEEVMPQA ENHDPDIKEH VNSLGENLKT LRLRLRRCHR FLPCENKSKA VEQVKNAFSK LQEKGVYKAM SEFDIFINYI EAYMTMKIQN. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Major immune regulatory cytokine that acts on many cells of the immune system where it has profound anti-inflammatory functions, limiting excessive tissue disruption caused by inflammation. Mechanistically, IL10 binds to its heterotetrameric receptor comprising IL10RA and IL10RB leading to JAK1 and STAT2-mediated phosphorylation of STAT3. Reference: "Comparative sequence analysis of cytokine genes from human and nonhuman primates." Villinger F.J., Brar S.S., Mayne A.E., Chikkala N., Ansari A.A. J. Immunol. 155:3946-3954(1995). Function: nan