Interleukin-1 (il1b), Danio rerio, recombinant

Organism: Danio rerio (Zebrafish) (Brachydanio rerio). Source: E.coli. Expression Region: 41-272aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: CDMHEGIRLE MWTSQHKMKQ LVNVIIALNR MKHIKPQSTE FGEKEVLDML MANVIQEREV NVVDSVPSYT KTKNVLQCTI CDQYKKSLVR SGGSPHLQAV TLRAGSSDLK VRFSMSTYAS PSAPATSAQP VCLGISKSNL YLACSPAEGS APHLVLKEIS GSLETIKAGD PNGYDQLLFF RKETGSSINT FESVKCPGWF ISTAYEDSQM VEMDRKDTER IINFELQDKV RI. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: n/a. Reference: "Pathogenesis and inflammatory response to Edwardsiella tarda infection in the zebrafish." Pressley M.E., Phelan P.E., Witten P.E., Mellon M.T., Kim C.H. Dev. Comp. Immunol. 29:501-513(2005). Function: nan