Appetite-regulating hormone (GHRL), partial, cat, recombinant

Organism: Felis catus (Cat) (Felis silvestris catus). Source: E.coli. Expression Region: 24-51aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-KSI-tagged. Target Protein Sequence: GSSFLSPEHQ KVQQRKESKK PPAKLQPR. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: [Ghrelin]: Ghrelin is the ligand for growth hormone secretagogue receptor type 1 (GHSR). Induces the release of growth hormone from the pituitary. Has an appetite-stimulating effect, induces adiposity and stimulates gastric acid secretion. Involved in growth regulation., [Obestatin]: Obestatin may be the ligand for GPR39. May have an appetite-reducing effect resulting in decreased food intake. May reduce gastric emptying activity and jejunal motility. Reference: "Purification and characterization of feline ghrelin and its possible role." Ida T., Miyazato M., Naganobu K., Nakahara K., Sato M., Lin X.Z., Kaiya H., Doi K., Noda S., Kubo A., Murakami N., Kangawa K. Domest. Anim. Endocrinol. 32:93-105(2007). Function: nan