Zinc finger protein 175 (ZNF175), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-711aa. Protein Length: Full Length. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: MPADVNLSQK PQVLGPEKQD GSCEASVSFE DVTVDFSREE WQQLDPAQRC LYRDVMLELY SHLFAVGYHI PNPEVIFRML KEKEPRVEEA EVSHQRCQER EFGLEIPQKE ISKKASFQKD MVGEFTRDGS WCSILEELRL DADRTKKDEQ NQIQPMSHSA FFNKKTLNTE SNCEYKDPGK MIRTRPHLAS SQKQPQKCCL FTESLKLNLE VNGQNESNDT EQLDDVVGSG QLFSHSSSDA CSKNIHTGET FCKGNQCRKV CGHKQSLKQH QIHTQKKPDG CSECGGSFTQ KSHLFAQQRI HSVGNLHECG KCGKAFMPQL KLSVYLTDHT GDIPCICKEC GKVFIQRSEL LTHQKTHTRK KPYKCHDCGK AFFQMLSLFR HQRTHSREKL YECSECGKGF SQNSTLIIHQ KIHTGERQYA CSECGKAFTQ KSTLSLHQRI HSGQKSYVCI ECGQAFIQKA HLIVHQRSHT GEKPYQCHNC GKSFISKSQL DIHHRIHTGE KPYECSDCGK TFTQKSHLNI HQKIHTGERH HVCSECGKAF NQKSILSMHQ RIHTGEKPYK CSECGKAFTS KSQFKEHQRI HTGEKPYVCT ECGKAFNGRS NFHKHQITHT RERPFVCYKC GKAFVQKSEL ITHQRTHMGE KPYECLDCGK SFSKKPQLKV HQRIHTGERP YVCSECGKAF NNRSNFNKHQ TTHTRDKSYK CSYSVKGFTK Q. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Down-regulates the expression of several chokine receptors. Interferes with HIV-1 replication by suppressing Tat-induced viral LTR promoter activity. Reference: The DNA sequence and biology of human chromosome 19.Grimwood J., Gordon L.A., Olsen A.S., Terry A., Schmutz J., Lamerdin J.E., Hellsten U., Goodstein D., Couronne O., Tran-Gyamfi M., Aerts A., Altherr M., Ashworth L., Bajorek E., Black S., Branscomb E., Caenepeel S., Carrano A.V. , Caoile C., Chan Y.M., Christensen M., Cleland C.A., Copeland A., Dalin E., Dehal P., Denys M., Detter J.C., Escobar J., Flowers D., Fotopulos D., Garcia C., Georgescu A.M., Glavina T., Gomez M., Gonzales E., Groza M., Hammon N., Hawkins T., Haydu L., Ho I., Huang W., Israni S., Jett J., Kadner K., Kimball H., Kobayashi A., Larionov V., Leem S.-H., Lopez F., Lou Y., Lowry S., Malfatti S., Martinez D., McCready P.M., Medina C., Morgan J., Nelson K., Nolan M., Ovcharenko I., Pitluck S., Pollard M., Popkie A.P., Predki P., Quan G., Ramirez L., Rash S., Retterer J., Rodriguez A., Rogers S., Salamov A., Salazar A., She X., Smith D., Slezak T., Solovyev V., Thayer N., Tice H., Tsai M., Ustaszewska A., Vo N., Wagner M., Wheeler J., Wu K., Xie G., Yang J., Dubchak I., Furey T.S., DeJong P., Dickson M., Gordon D., Eichler E.E., Pennacchio L.A., Richardson P., Stubbs L., Rokhsar D.S., Myers R.M., Rubin E.M., Lucas S.M.Nature 428:529-535(2004). Function: Down-regulates the expression of several chemokine receptors. Interferes with HIV-1 replication by suppressing Tat-induced viral LTR promoter activity.