Tryptophan--tRNA ligase, cytoplasmic (WARS), human, recombinant

Organism: Homo sapiens (Human). Source: Yeast. Expression Region: 2-471aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: PNSEPASLLE LFNSIATQGE LVRSLKAGNA SKDEIDSAVK MLVSLKMSYK AAAGEDYKAD CPPGNPAPTS NHGPDATEAE EDFVDPWTVQ TSSAKGIDYD KLIVRFGSSK IDKELINRIE RATGQRPHHF LRRGIFFSHR DMNQVLDAYE NKKPFYLYTG RGPSSEAMHV GHLIPFIFTK WLQDVFNVPL VIQMTDDEKY LWKDLTLDQA YSYAVENAKD IIACGFDINK TFIFSDLDYM GMSSGFYKNV VKIQKHVTFN QVKGIFGFTD SDCIGKISFP AIQAAPSFSN SFPQIFRDRT DIQCLIPCAI DQDPYFRMTR DVAPRIGYPK PALLHSTFFP ALQGAQTKMS ASDPNSSIFL TDTAKQIKTK VNKHAFSGGR DTIEEHRQFG GNCDVDVSFM YLTFFLEDDD KLEQIRKDYT SGAMLTGELK KALIEVLQPL IAEHQARRKE VTDEIVKEFM TPRKLSFDFQ. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression. Reference: "Human interferon gamma potently induces the synthesis of a 55-kDa protein (gamma 2) highly homologous to rabbit peptide chain release factor and bovine tryptophanyl-tRNA synthetase." Fleckner J., Rasmussen H.H., Justesen J. Proc. Natl. Acad. Sci. U.S.A. 88:11520-11524(1991). Function: Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.