Transcriptional enhancer factor TEF-4 (TEAD2), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 94-447aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: TRTRKQVSSH IQVLARRKSR EIQSKLKDQV SKDKAFQTMA TMSSAQLISA PSLQAKLGPT GPQASELFQF WSGGSGPPWN VPDVKPFSQT PFTLSLTPPS TDLPGYEPPQ ALSPLPPPTP SPPAWQARGL GTARLQLVEF SAFVEPPDAV DSYQRHLFVH ISQHCPSPGA PPLESVDVRQ IYDKFPEKKG GLRELYDRGP PHAFFLVKFW ADLNWGPSGE EAGAGGSISS GGFYGVSSQY ESLEHMTLTC SSKVCSFGKQ VVEKVETERA QLEDGRFVYR LLRSPMCEYL VNFLHKLRQL PERYMMNSVL ENFTILQVVT NRDTQELLLC TAYVFEVSTS ERGAQHHIYR LVRD. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3'). May be involved in the gene regulation of neural development. Binds to the M-CAT motif. Reference: "TEAD transcription factors mediate the function of TAZ in cell growth and epithelial-mesenchymal transition." Zhang H., Liu C.Y., Zha Z.Y., Zhao B., Yao J., Zhao S., Xiong Y., Lei Q.Y., Guan K.L. J. Biol. Chem. 284:13355-13362(2009). Function: nan