Toll-like receptor 7 (Tlr7), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 27-348aa. Protein Length: Partial. Tag Info: N-terminal GST-tagged. Target Protein Sequence: FRWFPKTLPC EVKVNIPEAH VIVDCTDKHL TEIPEGIPTN TTNLTLTINH IPSISPDSFR RLNHLEEIDL RCNCVPVLLG SKANVCTKRL QIRPGSFSGL SDLKALYLDG NQLLEIPQDL PSSLHLLSLE ANNIFSITKE NLTELVNIET LYLGQNCYYR NPCNVSYSIE KDAFLVMRNL KVLSLKDNNV TAVPTTLPPN LLELYLYNNI IKKIQENDFN NLNELQVLDL SGNCPRCYNV PYPCTPCENN SPLQIHDNAF NSLTELKVLR LHSNSLQHVP PTWFKNMRNL QELDLSQNYL AREIEEAKFL HFLPNLVELD FS. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Reference: UNC93B1 delivers nucleotide-sensing toll-like receptors to endolysosomes.Kim Y.M., Brinkmann M.M., Paquet M.E., Ploegh H.L.Nature 452:234-238(2008). Function: Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. TLR7 is a nucleotide-sensing TLR which is activated by single-stranded RNA. Acts via MYD88 and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response.