Synaptotagmin-1 (SYT1), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 99-416aa. Protein Length: Cytoplasmic Domain. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: KNAINMKDVK DLGKTMKDQA LKDDDAETGL TDGEEKEEPK EEEKLGKLQY SLDYDFQNNQ LLVGIIQAAE LPALDMGGTS DPYVKVFLLP DKKKKFETKV HRKTLNPVFN EQFTFKVPYS ELGGKTLVMA VYDFDRFSKH DIIGEFKVPM NTVDFGHVTE EWRDLQSAEK EEQEKLGDIC FSLRYVPTAG KLTVVILEAK NLKKMDVGGL SDPYVKIHLM QNGKRLKKKK TTIKKNTLNP YYNESFSFEV PFEQIQKVQV VVTVLDYDKI GKNDAIGKVF VGYNSTGAEL RHWSDMLANP RRPIAQWHTL QVEEEVDA. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May have a regulatory role in the mbrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca2+-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca2+-independent manner, these are neurexins, syntaxin and AP2. Reference: Identification of a human synaptotagmin-1 mutation that perturbs synaptic vesicle cycling.Baker K., Gordon S.L., Grozeva D., van Kogelenberg M., Roberts N.Y., Pike M., Blair E., Hurles M.E., Chong W.K., Baldeweg T., Kurian M.A., Boyd S.G., Cousin M.A., Raymond F.L.J. Clin. Invest. 0:0-0(2015). Function: May have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner, these are neurexins, syntaxin and AP2. Plays a role in dendrite formation by melanocytes