Sulfotransferase 1A3, Cytosolic, Recombinant, Human (SULT1A3)

Cytosolic sulfotransferases are a family of Phase II drug­metabolizing enzymes that catalyze the sulfation of many endogenous and xenobiotic substrates (1­3). They have important roles in the metabolism of many endogenous compounds including steroids, bile acids, thyroid hormones and monoamine neurotransmitters. They are widely distributed throughout the body and serve to inactivate and increase water­solubility of xenobiotics and therapeutic drugs. They are distinct from Golgi resident sulfotransferases by lacking N­ terminal signal­anchor domains and residing only in the cytoplasm. SULT1A3 catalyzes the sulfation of phenolic monoamines, such as dopamine, norepinephrine and serotonin, and phenolic and catecholic drugs (4, 5). SULT1A3 is also called catecholamine sulfotransferase. Recombinant enzyme corresponding to aa2-295 with an N-terminal Met and 6-His tag expressed in E. coli (P50224). , N-terminal Sequence Analysis: , Met , SDS-PAGE: 34kD, reducing conditions, Activity: Measured by its ability to transfer sulfate from PAPS to 1-Napthol. Specific Activity: >15pmoles/min/ug, as measured under the described conditions (see Protocol). Stability & Storage: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.