Sulfotransferase 1A1, Recombinant, Human (SULT1A1, Aryl sulfotransferase 1, HAST1/HAST2, OK/SW-cl.88

Cytosolic sulfotransferases catalyze the sulfonation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. They are distinct from Golgi resident sulfotransferases by the absence of transmembrane domains and are located in the cytoplasm. SULT1A1 is one of two phenol sulfotransferases with thermostable enzyme activity. The enzyme is more specific for sulfonation on catecholamines, phenolic drugs and neurotransmitters. Because of its ability to modify diverse promutagen and procarcinogen xenobiotics, it is implicated in a range of cancers. The crystal structure of the enzyme reveals that its active site is flexible and can accommodate diverse hydrophobic substrates with varying sizes, shapes and flexibility. Recombinant protein corresponding to aa2-295 of human Sulfotransferase 1A1 with N-terminal Met and 6-His tag expressed in E. coli (P50225). N-terminal Sequence Analysis: His, Predicted Molecular Mass: 35kD, SDS-PAGE: 34kD, reducing conditions, Activity: Measured by its ability to transfer sulfate from PAPS to 1­Napthol. Specific Activity: >30 pmol/min/ug, Endotoxin: <1.0 EU per 1ug (LAL). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.