Serpin A9 (SERPINA9), human, recombinant

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 24-417aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: ANAPSAYPRP SSTKSTPASQ VYSLNTDFAF RLYRRLVLET PSQNIFFSPV SVSTSLAMLS LGAHSVTKTQ ILQGLGFNLT HTPESAIHQG FQHLVHSLTV PSKDLTLKMG SALFVKKELQ LQANFLGNVK RLYEAEVFST DFSNPSIAQA RINSHVKKKT QGKVVDIIQG LDLLTAMVLV NHIFFKAKWE KPFHPEYTRK NFPFLVGEQV TVHVPMMHQK EQFAFGVDTE LNCFVLQMDY KGDAVAFFVL PSKGKMRQLE QALSARTLRK WSHSLQKRWI EVFIPRFSIS ASYNLETILP KMGIQNVFDK NADFSGIAKR DSLQVSKATH KAVLDVSEEG TEATAATTTK FIVRSKDGPS YFTVSFNRTF LMMITNKATD GILFLGKVEN PTKS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Protease inhibitor that inhibits trypsin and trypsin-like serine proteases. Inhibits plasmin and thrombin with lower efficiency. Reference: "Two newly characterized germinal center B-cell-associated genes, GCET1 and GCET2, have differential expression in normal and neoplastic B cells." Pan Z., Shen Y., Du C., Zhou G., Rosenwald A., Staudt L.M., Greiner T.C., McKeithan T.W., Chan W.C. Am. J. Pathol. 163:135-144(2003). Function: Protease inhibitor that inhibits trypsin and trypsin-like serine proteases (in vitro). Inhibits plasmin and thrombin with lower efficiency (in vitro).