Serine palmitoyltransferase 2 (SPTLC2), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 88-562aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: RDFLRYWRIE KCHHATEREE QKDFVSLYQD FENFYTRNLY MRIRDNWNRP ICSVPGARVD IMERQSHDYN WSFKYTGNII KGVINMGSYN YLGFARNTGS CQEAAAKVLE EYGAGVCSTR QEIGNLDKHE ELEELVARFL GVEAAMAYGM GFATNSMNIP ALVGKGCLIL SDELNHASLV LGARLSGATI RIFKHNNMQS LEKLLKDAIV YGQPRTRRPW KKILILVEGI YSMEGSIVRL PEVIALKKKY KAYLYLDEAH SIGALGPTGR GVVEYFGLDP EDVDVMMGTF TKSFGASGGY IGGKKELIDY LRTHSHSAVY ATSLSPPVVE QIITSMKCIM GQDGTSLGKE CVQQLAENTR YFRRRLKEMG FIIYGNEDSP VVPLMLYMPA KIGAFGREML KRNIGVVVVG FPATPIIESR ARFCLSAAHT KEILDTALKE IDEVGDLLQL KYSRHRLVPL LDRPFDETTY EETED. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC2-SPTSSB complex displays a preference for C18-CoA substrate. Reference: Initial characterization of the human central proteome.Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.BMC Syst. Biol. 5:17-17(2011). Function: Serine palmitoyltransferase (SPT). The heterodimer formed with LCB1/SPTLC1 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC2-SPTSSB complex displays a preference for C18-CoA substrate.