Semaphorin-3F (SEMA3F), human, recombinant

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 19-785aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-Myc-tagged. Target Protein Sequence: SFPTQDHLPA TPRVRLSFKE LKATGTAHFF NFLLNTTDYR ILLKDEDHDR MYVGSKDYVL SLDLHDINRE PLIIHWAASP QRIEECVLSG KDVNGECGNF VRLIQPWNRT HLYVCGTGAY NPMCTYVNRG RRAQATPWTQ TQAVRGRGSR ATDGALRPMP TAPRQDYIFY LEPERLESGK GKCPYDPKLD TASALINEEL YAGVYIDFMG TDAAIFRTLG KQTAMRTDQY NSRWLNDPSF IHAELIPDSA ERNDDKLYFF FRERSAEAPQ SPAVYARIGR ICLNDDGGHC CLVNKWSTFL KARLVCSVPG EDGIETHFDE LQDVFVQQTQ DVRNPVIYAV FTSSGSVFRG SAVCVYSMAD IRMVFNGPFA HKEGPNYQWM PFSGKMPYPR PGTCPGGTFT PSMKSTKDYP DEVINFMRSH PLMYQAVYPL QRRPLVVRTG APYRLTTIAV DQVDAADGRY EVLFLGTDRG TVQKVIVLPK DDQELEELML EEVEVFKDPA PVKTMTISSK RQQLYVASAV GVTHLSLHRC QAYGAACADC CLARDPYCAW DGQACSRYTA SSKRRSRRQD VRHGNPIRQC RGFNSNANKN AVESVQYGVA GSAAFLECQP RSPQATVKWL FQRDPGDRRR EIRAEDRFLR TEQGLLLRAL QLSDRGLYSC TATENNFKHV VTRVQLHVLG RDAVHAALFP PLSMSAPPPP GAGPPTPPYQ ELAQLLAQPE VGLIHQYCQG YWRHVPPSPR EAPGAPRSPE PQDQKKPRNR RHHPPDT. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May play a role in cell motility and cell adhesion. Reference: "Distinct 3p21.3 deletions in lung cancer and identification of a new human semaphorin."Roche J., Boldog F., Robinson M., Robinson L., Varella-Garcia M., Swanton M., Waggoner B., Fishel R., Franklin W., Gemmill R., Drabkin H.Oncogene 12:1289-1297(1996). Function: May play a role in cell motility and cell adhesion.