SARS-CoV-2 Papain-like Protease (recombinant)

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped positive-stranded RNA virus and the causative agent of COVID-19, a primarily respiratory illness characterized by fever, cough, and shortness of breath that can lead to life-threatening complications. The SARS-CoV-2 genome contains approximately 30 kilobases and 14 open reading frames (ORFs) that encode four structural proteins: spike, envelope, membrane, and nucleocapsid, as well as 16 non-structural proteins and 9 accessory factors. The SARS-CoV-2 papain-like protease (PLpro) is encoded within the non-structural protein 3 (nsp3) region of ORF1ab. It cleaves non-structural proteins 1-3, which, together with non-structural proteins 4-16, form the replicase complex. In addition, it reverses certain post-translational modifications, preferentially removing interferon-stimulated gene product 15 (ISG15), but also removing ubiquitin, from host proteins with both actions leading to suppression of the host innate immune response. SARS-CoV-2 PLpro decreases IFN-alpha-induced ISGylation of IFN regulatory factor 3 (IRF3) in A549 cells and inhibits the type I IFN response. Inhibition of SARS-CoV-2 PLpro activity reduces SARS-CoV-2 replication and viral particle release from infected cells in vitro and increases the level of IRF3 ISGylation. Cayman's SARS-CoV-2 Papain-like Protease (recombinant) protein can be used for enzyme activity assays. This protein consists of 324 amino acids and has a calculated molecular weight of 36.8 kDa.Synonyms: 2019-nCoV PLpro, COVID-19-PLpro, SARS-CoV-2 PLpro, Severe Acute Respiratory Syndrome Coronavirus 2 Papain-like Protease. Purity: >90% estimated by SDS-PAGE. Source: Active recombinant SARS-CoV-2 N-terminal His-tagged papain-like protease expressed in E. coli. Amino Acids: 1,564-1,880. MW: 36.8 kDa. Formulation: (Request formulation change), Lyophilized from sterile 20 mM Tris, pH 7.4, with 500 mM sodium chloride. Specific Activity: >80 pmols/min/µg.