Receptor-type tyrosine-protein phosphatase C (PTPRC), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 26-577aa. Protein Length: Extracellular Domain. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: QSPTPSPTGL TTAKMPSVPL SSDPLPTHTT AFSPASTFER ENDFSETTTS LSPDNTSTQV SPDSLDNASA FNTTGVSSVQ TPHLPTHADS QTPSAGTDTQ TFSGSAANAK LNPTPGSNAI SDVPGERSTA STFPTDPVSP LTTTLSLAHH SSAALPARTS NTTITANTSD AYLNASETTT LSPSGSAVIS TTTIATTPSK PTCDEKYANI TVDYLYNKET KLFTAKLNVN ENVECGNNTC TNNEVHNLTE CKNASVSISH NSCTAPDKTL ILDVPPGVEK FQLHDCTQVE KADTTICLKW KNIETFTCDT QNITYRFQCG NMIFDNKEIK LENLEPEHEY KCDSEILYNN HKFTNASKII KTDFGSPGEP QIIFCRSEAA HQGVITWNPP QRSFHNFTLC YIKETEKDCL NLDKNLIKYD LQNLKPYTKY VLSLHAYIIA KVQRNGSAAM CHFTTKSAPP SQVWNMTVSM TSDNSMHVKC RPPRDRNGPH ERYHLEVEAG NTLVRNESHK NCDFRVKDLQ YSTDYTFKAY FHNGDYPGEP FILHHSTSYN SK. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity. Reference: "Structural variants of human T200 glycoprotein (leukocyte-common antigen)." Ralph S.J., Thomas M.L., Morton C.C., Trowbridge I.S. EMBO J. 6:1251-1257(1987). Function: Protein tyrosine-protein phosphatase required for T-cell activation through the antigen receptor. Acts as a positive regulator of T-cell coactivation upon binding to DPP4. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits and dephosphorylates SKAP1 and FYN. Dephosphorylates LYN, and thereby modulates LYN activity (By similarity).