Prothrombin (F2), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 44-622aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: ANTFLEEVRK GNLERECVEE TCSYEEAFEA LESSTATDVF WAKYTACETA RTPRDKLAAC LEGNCAEGLG TNYRGHVNIT RSGIECQLWR SRYPHKPEIN STTHPGADLQ ENFCRNPDSS TTGPWCYTTD PTVRRQECSI PVCGQDQVTV AMTPRSEGSS VNLSPPLEQC VPDRGQQYQG RLAVTTHGLP CLAWASAQAK ALSKHQDFNS AVQLVENFCR NPDGDEEGVW CYVAGKPGDF GYCDLNYCEE AVEEETGDGL DEDSDRAIEG RTATSEYQTF FNPRTFGSGE ADCGLRPLFE KKSLEDKTER ELLESYIDGR IVEGSDAEIG MSPWQVMLFR KSPQELLCGA SLISDRWVLT AAHCLLYPPW DKNFTENDLL VRIGKHSRTR YERNIEKISM LEKIYIHPRY NWRENLDRDI ALMKLKKPVA FSDYIHPVCL PDRETAASLL QAGYKGRVTG WGNLKETWTA NVGKGQPSVL QVVNLPIVER PVCKDSTRIR ITDNMFCAGY KPDEGKRGDA CEGDSGGPFV MKSPFNNRWY QMGIVSWGEG CDRDGKYGFY THVFRLKKWI QKVIDQFGE. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Reference: "Nucleotide sequence of the gene for human prothrombin."Degen S.J.F., Davie E.W.Biochemistry 26:6165-6177(1987). Function: Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing.