Protein mono-ADP-ribosyltransferase PARP12 (PARP12), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-701aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: MAQAGVVGEV TQVLCAAGGA LELPELRRRL RMGLSADALE RLLRQRGRFV VAVRAGGAAA APERVVLAAS PLRLCRAHQG SKPGCVGLCA QLHLCRFMVY GACKFLRAGK NCRNSHSLTT EHNLSVLRTH GVDHLSYNEL CQLLFQNDPW LLPEICQHYN KGDGPHGSCA FQKQCIKLHI CQYFLQGECK FGTSCKRSHD FSNSENLEKL EKLGMSSDLV SRLPTIYRNA HDIKNKSSAP SRVPPLFVPQ GTSERKDSSG SVSPNTLSQE EGDQICLYHI RKSCSFQDKC HRVHFHLPYR WQFLDRGKWE DLDNMELIEE AYCNPKIERI LCSESASTFH SHCLNFNAMT YGATQARRLS TASSVTKPPH FILTTDWIWY WSDEFGSWQE YGRQGTVHPV TTVSSSDVEK AYLAYCTPGS DGQAATLKFQ AGKHNYELDF KAFVQKNLVY GTTKKVCRRP KYVSPQDVTT MQTCNTKFPG PKSIPDYWDS SALPDPGFQK ITLSSSSEEY QKVWNLFNRT LPFYFVQKIE RVQNLALWEV YQWQKGQMQK QNGGKAVDER QLFHGTSAIF VDAICQQNFD WRVCGVHGTS YGKGSYFARD AAYSHHYSKS DTQTHTMFLA RVLVGEFVRG NASFVRPPAK EGWSNAFYDS CVNSVSDPSI FVIFEKHQVY PEYVIQYTTS SKPSVTPSIL LALGSLFSSR Q. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins. Reference: "Towards a catalog of human genes and proteins: sequencing and analysis of 500 novel complete protein coding human cDNAs." Wiemann S., Weil B., Wellenreuther R., Gassenhuber J., Glassl S., Ansorge W., Boecher M., Bloecker H., Bauersachs S., Blum H., Lauber J., Duesterhoeft A., Beyer A., Koehrer K., Strack N., Mewes H.-W., Ottenwaelder B., Obermaier B. Poustka A. Genome Res. 11:422-435(2001). Function: nan