Protein-L-isoaspartate (D-aspartate) O-methyltransferase(Pcmt), Drosophila melanogaster, recombinant

Organism: Drosophila melanogaster (Fruit fly). Source: E.coli. Expression Region: 1-226aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: MAWRSVGANN EDLIRQLKDH GVIASDAVAQ AMKETDRKHY SPRNPYMDAP QPIGGGVTIS APHMHAFALE YLRDHLKPGA RILDVGSGSG YLTACFYRYI KAKGVDADTR IVGIEHQAEL VRRSKANLNT DDRSMLDSGQ LLIVEGDGRK GYPPNAPYNA IHVGAAAPDT PTELINQLAS GGRLIVPVGP DGGSQYMQQY DKDANGKVEM TRLMGVMYVP LTDLRS. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the methyl esterification of L-isoaspartyl and D-aspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins. Reference: "Structural organization and developmental expression of the protein isoaspartyl methyltransferase gene from Drosophila melanogaster." O'Connor M.B., Galus A., Hartenstine M., Magee M., Jackson F.R., O'Connor C.M. Insect Biochem. Mol. Biol. 27:49-54(1997). Function: nan