Prolyl endopeptidase (PREP), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-710aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: LSLQYPDVYR DETAVQDYHG HKICDPYAWL EDPDSEQTKA FVEAQNKITV PFLEQCPIRG LYKERMTELY DYPKYSCHFK KGKRYFYFYN TGLQNQRVLY VQDSLEGEAR VFLDPNILSD DGTVALRGYA FSEDGEYFAY GLSASGSDWV TIKFMKVDGA KELPDVLERV KFSCMAWTHD GKGMFYNSYP QQDGKSDGTE TSTNLHQKLY YHVLGTDQSE DILCAEFPDE PKWMGGAELS DDGRYVLLSI REGCDPVNRL WYCDLQQESS GIAGILKWVK LIDNFEGEYD YVTNEGTVFT FKTNRQSPNY RVINIDFRDP EESKWKVLVP EHEKDVLEWI ACVRSNFLVL CYLHDVKNIL QLHDLTTGAL LKTFPLDVGS IVGYSGQKKD TEIFYQFTSF LSPGIIYHCD LTKEELEPRV FREVTVKGID ASDYQTVQIF YPSKDGTKIP MFIVHKKGIK LDGSHPAFLY GYGGFNISIT PNYSVSRLIF VRHMGGILAV ANIRGGGEYG ETWHKGGILA NKQNCFDDFQ CAAEYLIKEG YTSPKRLTIN GGSNGGLLVA ACANQRPDLF GCVIAQVGVM DMLKFHKYTI GHAWTTDYGC SDSKQHFEWL VKYSPLHNVK LPEADDIQYP SMLLLTADHD DRVVPLHSLK FIATLQYIVG RSRKQSNPLL IHVDTKAGHG AGKPTAKVIE EVSDMFAFIA RCLNVDWIP. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Reference: "Pyrrolidinyl pyridone and pyrazinone analogues as potent inhibitors of prolyl oligopeptidase (POP)." Haffner C.D., Diaz C.J., Miller A.B., Reid R.A., Madauss K.P., Hassell A., Hanlon M.H., Porter D.J., Becherer J.D., Carter L.H. Bioorg. Med. Chem. Lett. 18:4360-4363(2008). Function: nan