PLA2G7, human recombinant protein

Human PLA2G7, (GenBank Accession No. NM_005084), (amino acids 22-441(end)), with C-terminal His-tag, MW=49.2 kDa, expressed in a mammalian cell expression system. Assay Conditions: Enzyme reaction is conducted using liposomally incorporated Red/Green BODIPY PC-A2 (5 ?M, Invitrogen) as a substrate in a reaction buffer (50 mM Tris, pH 8.8, 100 mM NaCl, 1 mM CaCl2). Reaction progress was monitored at ex480/em533 continuously at room temperature. Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.