Phospholipase A2, Apis mellifera, recombinant

Organism: Apis mellifera (Honeybee). Source: E.coli. Expression Region: 34-167aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: IIYPGTLWCG HGNKSSGPNE LGRFKHTDAC CRTHDMCPDV MSAGESKHGL TNTASHTRLS CDCDDKFYDC LKNSADTISS YFVGKMYFNL IDTKCYKLEH PVTGCGERTE GRCLHYTVDK SKPKVYQWFD LRKY. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Reference: "Crystal structure of bee-venom phospholipase A2 in a complex with a transition-state analogue." Scott D.L., Otwinowski Z., Gelb M.H., Sigler P.B. Science 250:1563-1566(1990). Function: nan