Phosphatidylserine decarboxylase proenzyme, mitochondrial (PISD), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1-375aa. Protein Length: Full Length of Isoform 2. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: MMCQSEARQG PELRAAKWLH FPQLALRRRL GQLSCMSRPA LKLRSWPLTV LYYLLPFGAL RPLSRVGWRP VSRVALYKSV PTRLLSRAWG RLNQVELPHW LRRPVYSLYI WTFGVNMKEA AVEDLHHYRN LSEFFRRKLK PQARPVCGLH SVISPSDGRI LNFGQVKNCE VEQVKGVTYS LESFLGPRMC TEDLPFPPAA SCDSFKNQLV TREGNELYHC VIYLAPGDYH CFHSPTDWTV SHRRHFPGSL MSVNPGMARW IKELFCHNER VVLTGDWKHG FFSLTAVGAT NVGSIRIYFD RDLHTNSPRH SKGSYNDFSF VTHTNREGVP MRKGEHLGEF NLGSTIVLIF EAPKDFNFQL KTGQKIRFGE ALGSL. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: n/a. Reference: A genome annotation-driven approach to cloning the human ORFeome.Collins J.E., Wright C.L., Edwards C.A., Davis M.P., Grinham J.A., Cole C.G., Goward M.E., Aguado B., Mallya M., Mokrab Y., Huckle E.J., Beare D.M., Dunham I.Genome Biol. 5:R84.1-R84.11(2004). Function: Catalyzes the formation of phosphatidylethanolamine (PtdEtn) from phosphatidylserine (PtdSer). Plays a central role in phospholipid metabolism and in the interorganelle trafficking of phosphatidylserine.