PDK1, active, Recombinant, Human (PDPK1, PI?P3 Dependent Kinase, 3a Phosphoinositide dependent Prote

Recombinant full-length house PDK1 was expressed by baculovirus in Sf9 insect cells using a N-terminal His tag. The gene accession number is NM_002613. Specific Activity: As Reported , Assay Conditions: Assay was done in Kinase buffer containing 0.2mM DTT using PDKtide substrate (0.1mg/ml) and 20uM ATP. Reaction was done at 30ºC fro 45 min. Amount of ATP transferred was measured using Kinase-Glo reagent. Applications: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: Aliquot to avoid repeated freezing and thawing and store at -70°C. Aliquots are stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.