P2Y purinoceptor 12 (P2ry12), mouse, recombinant

Organism: Mus musculus (Mouse). Source: in vitro E.coli expression system. Expression Region: 1-347aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: MDVPGVNTTS ANTTFSPGTS TLCVRDYKIT QVLFPLLYTV LFFAGLITNS LAMRIFFQIR SKSNFIIFLK NTVISDLLMI LTFPFKILSD AKLGAGPLRT LVCQVTSVTF YFTMYISISF LGLITIDRYL KTTRPFKTSS PSNLLGAKIL SVVIWAFMFL ISLPNMILTN RRPKDKDVTK CSFLKSEFGL VWHEIVNYIC QVIFWINFLI VIVCYSLITK ELYRSYVRTR GSAKVPKKKV NVKVFIIIAV FFICFVPFHF ARIPYTLSQT RAVFDCSAEN TLFYVKESTL WLTSLNACLD PFIYFFLCKS FRNSLTSMLR CSNSTSTSGT NKKKGQEGGE PSEETPM. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Receptor for ADP and ATP coupled to G-proteins that inhibit the adenylyl cyclase second messenger system. Required for normal platelet aggregation and blood coagulation. Reference: "P2Y12 regulates platelet adhesion/activation, thrombus growth, and thrombus stability in injured arteries." Andre P., Delaney S.M., LaRocca T., Vincent D., DeGuzman F., Jurek M., Koller B., Phillips D.R., Conley P.B. J. Clin. Invest. 112:398-406(2003). Function: nan