P2Y purinoceptor 1 (P2ry1), mouse, recombinant

Organism: Mus musculus (Mouse). Source: in vitro E.coli expression system. Expression Region: 1-373aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: MTEVPWSVVP NGTDAAFLAG LGSLWGNSTV ASTAAVSSSF QCALTKTGFQ FYYLPAVYIL VFIIGFLGNS VAIWMFVFHM KPWSGISVYM FNLALADFLY VLTLPALIFY YFNKTDWIFG DAMCKLQRFI FHVNLYGSIL FLTCISAHRY SGVVYPLKSL GRLKKKNAIY VSVLVWLIVV VAISPILFYS GTGTRKNKTV TCYDTTSNDY LRSYFIYSMC TTVAMFCIPL VLILGCYGLI VKALIYNDLD NSPLRRKSIY LVIIVLTVFA VSYIPFHVMK TMNLRARLDF QTPEMCDFND RVYATYQVTR GLASLNSCVD PILYFLAGDT FRRRLSRATR KASRRSEANL QSKSEEMTLN ILSEFKQNGD TSL. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Receptor for extracellular adenine nucleotides such as ADP. In platelets, binding to ADP leads to mobilization of intracellular calcium ions via activation of phospholipase C, a change in platelet shape, and ultimately platelet aggregation. Reference: "Defective platelet aggregation and increased resistance to thrombosis in purinergic P2Y(1) receptor-null mice." Leon C., Hechler B., Freund M., Eckly A., Vial C., Ohlmann P., Dierich A., LeMeur M., Cazenave J.-P., Gachet C. J. Clin. Invest. 104:1731-1737(1999). Function: nan