P-Selectin/CD62P (mouse, recombinant)

P-selectin, also known as CD62P, is a glycoprotein and cell adhesion molecule that is encoded by Selp in mice. It is a homodimer and is composed of an N-terminal calcium-dependent lectin domain that recognizes glycoproteins, an EGF-like domain, nine consensus repeats, a transmembrane domain, and an intracellular C-terminal tail. P-selectin is expressed in platelets, endothelial cells, and macrophages, is stored in alpha-granules of platelets, and localizes to the plasma membrane upon platelet activation. It also exists as a soluble form that results from alternative splicing of SELP. P-selectin is involved in tethering and rolling of leukocytes during inflammation and tissue healing mediated by binding to P-selectin glycoprotein ligand-1 (PSGL-1), a mucin expressed on the surface of leukocytes. Selp knockout delays fatty streak formation and progression to the fibrous plaque stage in LDL receptor-deficient mice fed an atherogenic diet. It also delays aortic lesion progression in ApoE-/- mice. Cayman's P-Selectin/CD62P (mouse, recombinant) protein can be used for cell-based assays. This protein consists of 679 amino acids, has a calculated molecular weight of 74 kDa, and a predicted N-terminus of Trp42 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is 116 kDa due to glycosylation.Synonyms: CD62 Antigen-like Family Member P, GMP140, Granule Membrane Protein 140, PADGEM, Platelet Activation-dependent Granule-external Membrane Protein, Platelet-selectin, SELP. Purity: >95% estimated by SDS-PAGE. Endotoxin Testing: <1.0 EU/µg determined by the LAL endotoxin assay. Source: Active recombinant C-terminal His-tagged P-selectin expressed in HEK293 cells. Amino Acids: 42-709. MW: 74 kDa. Formulation: (Request formulation change), Lyophilized from sterile PBS, pH 7.4. UniProt Accession #: Q01102.