Neural cell adhesion molecule L1 (L1CAM), partial (Active), human, recombinant

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 20-1120aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: IQIPEEYEGH HVMEPPVITE QSPRRLVVFP TDDISLKCEA SGKPEVQFRW TRDGVHFKPK EELGVTVYQS PHSGSFTITG NNSNFAQRFQ GIYRCFASNK LGTAMSHEIR LMAEGAPKWP KETVKPVEVE EGESVVLPCN PPPSAEPLRI YWMNSKILHI KQDERVTMGQ NGNLYFANVL TSDNHSDYIC HAHFPGTRTI IQKEPIDLRV KATNSMIDRK PRLLFPTNSS SHLVALQGQP LVLECIAEGF PTPTIKWLRP SGPMPADRVT YQNHNKTLQL LKVGEEDDGE YRCLAENSLG SARHAYYVTV EAAPYWLHKP QSHLYGPGET ARLDCQVQGR PQPEVTWRIN GIPVEELAKD QKYRIQRGAL ILSNVQPSDT MVTQCEARNR HGLLLANAYI YVVQLPAKIL TADNQTYMAV QGSTAYLLCK AFGAPVPSVQ WLDEDGTTVL QDERFFPYAN GTLGIRDLQA NDTGRYFCLA ANDQNNVTIM ANLKVKDATQ ITQGPRSTIE KKGSRVTFTC QASFDPSLQP SITWRGDGRD LQELGDSDKY FIEDGRLVIH SLDYSDQGNY SCVASTELDV VESRAQLLVV GSPGPVPRLV LSDLHLLTQS QVRVSWSPAE DHNAPIEKYD IEFEDKEMAP EKWYSLGKVP GNQTSTTLKL SPYVHYTFRV TAINKYGPGE PSPVSETVVT PEAAPEKNPV DVKGEGNETT NMVITWKPLR WMDWNAPQVQ YRVQWRPQGT RGPWQEQIVS DPFLVVSNTS TFVPYEIKVQ AVNSQGKGPE PQVTIGYSGE DYPQAIPELE GIEILNSSAV LVKWRPVDLA QVKGHLRGYN VTYWREGSQR KHSKRHIHKD HVVVPANTTS VILSGLRPYS SYHLEVQAFN GRGSGPASEF TFSTPEGVPG HPEALHLECQ SNTSLLLRWQ PPLSHNGVLT GYVLSYHPLD EGGKGQLSFN LRDPELRTHN LTDLSPHLRY RFQLQATTKE GPGEAIVREG GTMALSGISD FGNISATAGE NYSVVSWVPK EGQCNFRFHI LFKALGEEKG GASLSPQYVS YNQSSYTQWD LQPDTDYEIH LFKERMFRHQ MAVKTNGTGR VRLPPAGFAT E. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized L1CAM at 2 µg/ml can bind Anti-L1CAM Rabbit Monoclonal Antibody (CSB-RA595071A0HU), the EC50 is 5.384-9.380 ng/ml. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: n/a. Reference: n/a. Function: nan