Neural cell adhesion molecule 1 (Ncam1), partial, rat, recombinant

Organism: Rattus norvegicus (Rat). Source: E.coli. Expression Region: 20-721aa. Protein Length: Extracellular Domain. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: LQVDIVPSQG EISVGESKFF LCQVAGDAKD KDISWFSPNG EKLSPNQQRI SVVWNDDDSS TLTIYNANID DAGIYKCVVT AEDGTQSEAT VNVKIFQKLM FKNAPTPQEF KEGEDAVIVC DVVSSLPPTI IWKHKGRDVI LKKDVRFIVL SNNYLQIRGI KKTDEGTYRC EGRILARGEI NFKDIQVIVN VPPTVQARQS IVNATANLGQ SVTLVCDADG FPEPTMSWTK DGEPIENEEE DDEKHIFSDD SSELTIRNVD KNDEAEYVCI AENKAGEQDA SIHLKVFAKP KITYVENQTA MELEEQVTLT CEASGDPIPS ITWRTSTRNI SSEEKASWTR PEKQETLDGH MVVRSHARVS SLTLKSIQYT DAGEYICTAS NTIGQDSQSM YLEVQYAPKL QGPVAVYTWE GNQVNITCEV FAYPSATISW FRDGQLLPSS NYSNIKIYNT PSASYLEVTP DSENDFGNYN CTAVNRIGQE SLEFILVQAD TPSSPSIDRV EPYSSTAQVQ FDEPEATGGV PILKYKAEWK SLGEEAWHSK WYDAKEANME GIVTIMGLKP ETRYAVRLAA LNGKGLGEIS AATEFKTQPV REPSAPKLEG QMGEDGNSIK VNLIKQDDGG SPIRHYLVKY RALASEWKPE IRLPSGSDHV MLKSLDWNAE YEVYVVAENQ QGKSKAAHFV FRTSAQPTAI PANGSPTAGL ST. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc. Reference: "Expression of the unique NCAM VASE exon is independently regulated in distinct tissues during development." Small S.J., Akeson R. J. Cell Biol. 111:2089-2096(1990). Function: This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc.