Nectin-4 (NECTIN4), partial (Active), human, recombinant

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 32-349aa. Protein Length: Partial. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: GELETSDVVT VVLGQDAKLP CFYRGDSGEQ VGQVAWARVD AGEGAQELAL LHSKYGLHVS PAYEGRVEQP PPPRNPLDGS VLLRNAVQAD EGEYECRVST FPAGSFQARL RLRVLVPPLP SLNPGPALEE GQGLTLAASC TAEGSPAPSV TWDTEVKGTT SSRSFKHSRS AAVTSEFHLV PSRSMNGQPL TCVVSHPGLL QDQRITHILH VSFLAEASVR GLEDQNLWHI GREGAMLKCL SEGQPPPSYN WTRLDGPLPS GVRVDGDTLG FPPLTTEHSG IYVCHVSNEF SSRDSQVTVD VLDPQEDSGK QVDLVSAS. Purity: Greater than 95% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized NECTIN4 at 2 µg/mL can bind anti-NECTIN4 antibody(CSB-RA822274A0HU)(enfortumab vedotin-like), the EC50 is 0.6029-0.7837 ng/mL. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells. (Microbial infection) Acts as a receptor for measles virus. Reference: n/a. Function: nan