NAD-dependent protein deacetylase (Sirt3), rat, recombinant

Organism: Rattus norvegicus (Rat). Source: E.coli. Expression Region: 1-257aa. Protein Length: Full Length. Tag Info: N-terminal 10xHis-tagged and C-terminal Myc-tagged. Target Protein Sequence: MVGAGISTPS GIPDFRSPGS GLYSNLQQYD IPYPEAIFEL GFFFHNPKPF FTLAKELYPG HYRPNVAHYF LRLLHDKELL LRLYTQNIDG LERASGIPAS KLVEAHGSFV SATCTVCRRS FPGEDIRADV MADRVPRCPV CTGVVKPDIV FFGEQLPARF LLHVADFALA DLLLILGTSL EVEPFASLSE SVQKSVPRLL INRDLVGSFA LSPRRKDVVQ LGDVVQGVER LVDLLGWTQE LQDLIQRENG KLDGQDG. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: NAD-dependent protein deacetylase. Reference: "Maternal obesity during gestation impairs fatty acid oxidation and mitochondrial SIRT3 expression in rat offspring at weaning." Borengasser S.J., Lau F., Kang P., Blackburn M.L., Ronis M.J., Badger T.M., Shankar K. PLoS ONE 6:e24068-e24068(2011). Function: nan