Murine RNase Inhibitor, GMP-Grade DMF #038031

Product Description: RNase inhibitors are essential to protect RNA from RNase contamination. It inhibits the enzymatic activity of RNases by noncovalently binding to the active site. Murine RNase inhibitor is a recombinant RNase inhibitor of murine origin expressed and purified in E.coli. It specifically inactivates RNases such as RNase A, RNase B, and RNase C by binding covalently with RNase to form a 1:1 complex. Murine RNase Inhibitor is compatible with RNA synthesis, RT-PCR and other RNA-related reactions. This product has been filed with the FDA Drug Master Files (DMF) and is assigned DMF #038031. Application: In Vitro Transcription, RT-PCR, cDNA synthesis. Concentration: 120U/µL. Unit Definition: One unit is defined as the amount of Murine RNase Inhibitor required to inhibit the activity of 5ng of RNase A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2', 3'-cyclic monophosphate by RNase A. Source: Expressed in an E.coli strain with the RNase inhibitor gene from mouse. Quality Standards: Activity (Ultraviolet spectrophotometry): > 120 kU/mL, Purity (SEC-HPLC): > 95%, Residual Endonuclease: Negative, Residual Exonuclease: Negative, Residual RNase: Negative, Residual Protease: Negative, Endotoxin: <= 10 EU/mL, Residual Host Cell DNA: <= 100 pg/mg, Residual Host Protein: <= 50 ng/mg, Bioburden: <= 1 CFU/10mL.