Mpro [3CLpro] (SARS-CoV-2) (rec.) (His)

Mpro (Papain-like Protease) (aa 1540-1841) (Accession Nr. P0C6U8) is fused at the N-terminus to a His-tag. 3CLpro recognizes the peptide sequence LQ[S/A/G] where it cleaves c-terminal to the amino acid glutamine (use product SBB-PS0130, KTSAVLQ-Rh110-Glu as universal substrate). The protease 3CLpro is a potential drug target for coronavirus infections due to its essential role in processing the polyproteins that are translated from the viral RNA. The X-ray structures of the unliganded SARS-CoV-2 protease 3CLpro and its complex with an alpha-ketoamide inhibitor provides a basis for design of a-ketoamide inhibitors. This SARS Coronavirus recombinant 3CLpro is N-terminally His10-tagged and expressed in E.coli. 3CLpro recognizes the peptide sequence LQ[S/A/G] where it cleaves C-terminal to the amino acid glutamine. Working concentrations of this enzyme range from 10 to 100 nM using KTSAVLQ-Rh110 (SBB-PS0130) as substrate.