MIS RII, Fc Chimera, Recombinant, Human (Mullerian Inhibiting Substance Type II Teceptor, AMHRII, an

Human MIS RII (Mullerian inhibiting substance type II receptor), also known as AMHRII (anti-Mullerian hormone type II receptor), is an 82kD serine/threonine receptor with a single transmembrane domain that belongs to the family of type II receptors of the TGF-Beta superfamily (1). The MIS RII precursor is 573 amino acids (aa) in length, with a 17 aa signal sequence, a 127 aa extracellular region that also contains two potential N-linked glycosylation sites, a 26 aa transmembrane region, and a 403 aa cytoplasmic region that contains the serine/threonine kinase domain (1). Human MIS RII shares 82%, 78%, and 77% aa sequence identity with rabbit, mouse, and rat MIS RII, respectively. It is expressed in the mesenchyme surrounding the fetal Mullerian duct, in fetal and postnatal granulosa cells, and in Sertoli cells (1-6). MIS RII is a receptor for Mullerian inhibitor substance (MIS), also known as anti-Mullerian hormone (AMH), which is responsible for regression of the Mullerian duct, the anlagen of the uterus, Fallopian tubes, and upper vagina in male fetuses (1-6). Mutations in MIS RII result in persistent Mullerian duct syndrome (PMDS), an extremely rare form of pseudohermaphroditism (5, 6), Source: A DNA sequence encoding the extracellular domain of Human MIS RII (Pro 18-Ser 144, Accession # Q16671) was fused to the Fc region of human IgG1 via a peptide linker (DIEGMRD). The chimeric protein was expressed in a mouse myeloma cell line, NS0. Structure/Form: Disulfide-linked homodimer Predicted Molecular Mass: 40.2 kDa (monomer). In SDS-PAGE (reducing conditions), migrates at 60-65kD as a result of glycosylation , Activity: Measured by its ability to bind rhMIS in a functional ELISA. Applications: Suitable for use in ELISA. Other applications not tested. Storage and Stability: Lyophilized powder may be stored at -20°C or lower. Powder is stable for 12 months at -20°C or lower. Reconstituted product under sterile conditions is stable for 1 month at 4°C or for 3 months at -20°C or lower. ,