Microtubule-associated protein 2 (Map2), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 213-559aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: GMEGEKLPPV PFAQTFGTNL EDRKQSTEPS IVMPSIGLSA EPPAPKEPKD WFIEMPTESK KDEWGLAAPI SPGPLTPMRE KDVLEDIPRW EGKQFDSPMP SPFHGGSFTL PLDTMKNERV SEGPRPFAPV FFQSDDKVSL QDPSALATSK ESSKDEEPLK DKADKVADVS ISEVTTLLGN VHSPVVEGYV GENISGEVKV TTDQEKKETS APSVQEPTLT ETEPQTKLDE KSTVSIEEAV AKKEESFKLR DDKTGVIQTS TEQSFSKEDQ KGQEHTIDEL KQDSFPISLE QAVTDAAMTS KTLGKVTSEP EAVSERREIQ GLFEEKTADK NKLEGAGSAT IAEVEMP. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also se to have a stiffening effect on microtubules. Reference: Qualitative and quantitative analyses of protein phosphorylation in naive and stimulated mouse synaptosomal preparations.Munton R.P., Tweedie-Cullen R., Livingstone-Zatchej M., Weinandy F., Waidelich M., Longo D., Gehrig P., Potthast F., Rutishauser D., Gerrits B., Panse C., Schlapbach R., Mansuy I.M.Mol. Cell. Proteomics 6:283-293(2007). Function: The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.