Methylmalonyl-CoA mutase, mitochondrial (MMUT), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 33-750aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: LHQQQPLHPE WAALAKKQLK GKNPEDLIWH TPEGISIKPL YSKRDTMDLP EELPGVKPFT RGPYPTMYTF RPWTIRQYAG FSTVEESNKF YKDNIKAGQQ GLSVAFDLAT HRGYDSDNPR VRGDVGMAGV AIDTVEDTKI LFDGIPLEKM SVSMTMNGAV IPVLANFIVT GEEQGVPKEK LTGTIQNDIL KEFMVRNTYI FPPEPSMKII ADIFEYTAKH MPKFNSISIS GYHMQEAGAD AILELAYTLA DGLEYSRTGL QAGLTIDEFA PRLSFFWGIG MNFYMEIAKM RAGRRLWAHL IEKMFQPKNS KSLLLRAHCQ TSGWSLTEQD PYNNIVRTAI EAMAAVFGGT QSLHTNSFDE ALGLPTVKSA RIARNTQIII QEESGIPKVA DPWGGSYMME CLTNDVYDAA LKLINEIEEM GGMAKAVAEG IPKLRIEECA ARRQARIDSG SEVIVGVNKY QLEKEDAVEV LAIDNTSVRN RQIEKLKKIK SSRDQALAER CLAALTECAA SGDGNILALA VDASRARCTV GEITDALKKV FGEHKANDRM VSGAYRQEFG ESKEITSAIK RVHKFMEREG RRPRLLVAKM GQDGHDRGAK VIATGFADLG FDVDIGPLFQ TPREVAQQAV DADVHAVGIS TLAAGHKTLV PELIKELNSL GRPDILVMCG GVIPPQDYEF LFEVGVSNVF GPGTRIPKAA VQVLDDIEKC LEKKQQSV. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Involved in the degradation of several amino acids, odd-chain fatty acids and cholesterol via propionyl-CoA to the tricarboxylic acid cycle. MCM has different functions in other species. Reference: "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome." Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H. J. Proteomics 96:253-262(2014). Function: Involved in the degradation of several amino acids, odd-chain fatty acids and cholesterol via propionyl-CoA to the tricarboxylic acid cycle. MCM has different functions in other species.