Matrix metalloproteinase-9 (MMP9), partial, human, recombinant

Organism: Homo sapiens (Human). Source: Yeast. Expression Region: 107-707aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: FQTFEGDLKW HHHNITYWIQ NYSEDLPRAV IDDAFARAFA LWSAVTPLTF TRVYSRDADI VIQFGVAEHG DGYPFDGKDG LLAHAFPPGP GIQGDAHFDD DELWSLGKGV VVPTRFGNAD GAACHFPFIF EGRSYSACTT DGRSDGLPWC STTANYDTDD RFGFCPSERL YTQDGNADGK PCQFPFIFQG QSYSACTTDG RSDGYRWCAT TANYDRDKLF GFCPTRADST VMGGNSAGEL CVFPFTFLGK EYSTCTSEGR GDGRLWCATT SNFDSDKKWG FCPDQGYSLF LVAAHEFGHA LGLDHSSVPE ALMYPMYRFT EGPPLHKDDV NGIRHLYGPR PEPEPRPPTT TTPQPTAPPT VCPTGPPTVH PSERPTAGPT GPPSAGPTGP PTAGPSTATT VPLSPVDDAC NVNIFDAIAE IGNQLYLFKD GKYWRFSEGR GSRPQGPFLI ADKWPALPRK LDSVFEERLS KKLFFFSGRQ VWVYTGASVL GPRRLDKLGL GADVAQVTGA LRSGRGKMLL FSGRRLWRFD VKAQMVDPRS ASEVDRMFPG VPLDTHDVFQ YREKAYFCQD RFYWRVSSRS ELNQVDQVGY VTYDILQCPE D. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: May play an essential role in local proteolysis of the Extracellular domain matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-, -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide. Reference: SV40-transformed human lung fibroblasts secrete a 92-KDA type IV collagenase which is identical to that secreted by normal human macrophages.Wilhelm S.M., Collier I.E., Marmer B.L., Eisen A.Z., Grant G.A., Goldberg G.I.J. Biol. Chem. 264:17213-17221(1989). Function: May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-, -Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.