Lysine-specific demethylase 6B (KDM6B), partial, human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 1176-1505aa. Protein Length: Partial. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: LPREKLNPPT PSIYLESKRD AFSPVLLQFC TDPRNPITVI RGLAGSLRLN LGLFSTKTLV EASGEHTVEV RTQVQQPSDE NWDLTGTRQI WPCESSRSHT TIAKYAQYQA SSFQESLQEE KESEDEESEE PDSTTGTPPS SAPDPKNHHI IKFGTNIDLS DAKRWKPQLQ ELLKLPAFMR VTSTGNMLSH VGHTILGMNT VQLYMKVPGS RTPGHQENNN FCSVNINIGP GDCEWFAVHE HYWETISAFC DRHGVDYLTG SWWPILDDLY ASNIPVYRFV QRPGDLVWIN AGTVHWVQAT GWCNNIAWNV GPLTAYQYQL ALERYEWNEV. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation. Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression by acting as a link between T-box factors and the SMARCA4-containing SWI/SNF remodeling complex. Reference: "The histone H3 lysine-27 demethylase Jmjd3 links inflammation to inhibition of polycomb-mediated gene silencing." De Santa F., Totaro M.G., Prosperini E., Notarbartolo S., Testa G., Natoli G. Cell 130:1083-1094(2007). Function: nan