Lanosterol synthase (LSS), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 2-732aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 10xHis-tagged. Target Protein Sequence: TEGTCLRRRG GPYKTEPATD LGRWRLNCER GRQTWTYLQD ERAGREQTGL EAYALGLDTK NYFKDLPKAH TAFEGALNGM TFYVGLQAED GHWTGDYGGP LFLLPGLLIT CHVARIPLPA GYREEIVRYL RSVQLPDGGW GLHIEDKSTV FGTALNYVSL RILGVGPDDP DLVRARNILH KKGGAVAIPS WGKFWLAVLN VYSWEGLNTL FPEMWLFPDW APAHPSTLWC HCRQVYLPMS YCYAVRLSAA EDPLVQSLRQ ELYVEDFASI DWLAQRNNVA PDELYTPHSW LLRVVYALLN LYEHHHSAHL RQRAVQKLYE HIVADDRFTK SISIGPISKT INMLVRWYVD GPASTAFQEH VSRIPDYLWM GLDGMKMQGT NGSQIWDTAF AIQALLEAGG HHRPEFSSCL QKAHEFLRLS QVPDNPPDYQ KYYRQMRKGG FSFSTLDCGW IVSDCTAEAL KAVLLLQEKC PHVTEHIPRE RLCDAVAVLL NMRNPDGGFA TYETKRGGHL LELLNPSEVF GDIMIDYTYV ECTSAVMQAL KYFHKRFPEH RAAEIRETLT QGLEFCRRQQ RADGSWEGSW GVCFTYGTWF GLEAFACMGQ TYRDGTACAE VSRACDFLLS RQMADGGWGE DFESCEERRY LQSAQSQIHN TCWAMMGLMA VRHPDIEAQE RGVRCLLEKQ LPNGDWPQEN IAGVFNKSCA ISYTSYRNIF PIWALGRFSQ LYPERALAGH P. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Key enzyme in the cholesterol biosynthesis pathway. Catalyzes the cyclization of (S)-2,3 oxidosqualene to lanosterol, a reaction that forms the sterol nucleus (PubMed:14766201, PubMed:7639730, PubMed:26200341). Through the production of lanosterol may regulate lens protein aggregation and increase transparency (PubMed:26200341). Reference: "Structure of the human lanosterol synthase gene and its analysis as a candidate for holoprosencephaly." Roessler E., Mittaz L., Du Y., Scott H.S., Chang J., Rossier C., Guipponi M., Matsuda S.P., Muenke M., Antonarakis S.E. Hum. Genet. 105:489-495(1999). Function: nan