Killer cell immunoglobulin-like receptor 3DL2 (KIR3DL2), partial (Active), human, recombinant

Organism: Homo sapiens (Human). Source: Mammalian cell. Expression Region: 22-340aa. Protein Length: Partial. Tag Info: C-terminal 10xHis-tagged. Target Protein Sequence: LMGGQDKPFL SARPSTVVPR GGHVALQCHY RRGFNNFMLY KEDRSHVPIF HGRIFQESFI MGPVTPAHAG TYRCRGSRPH SLTGWSAPSN PLVIMVTGNH RKPSLLAHPG PLLKSGETVI LQCWSDVMFE HFFLHREGIS EDPSRLVGQI HDGVSKANFS IGPLMPVLAG TYRCYGSVPH SPYQLSAPSD PLDIVITGLY EKPSLSAQPG PTVQAGENVT LSCSSWSSYD IYHLSREGEA HERRLRAVPK VNRTFQADFP LGPATHGGTY RCFGSFRALP CVWSNSSDPL LVSVTGNPSS SWPSPTEPSS KSGICRHLH. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Less than 1.0 EU/ug as determined by LAL method. Biological Activity: Measured by its binding ability in a functional ELISA. Immobilized Human KIR3DL2 at 2 µg/mL can bind Anti-KIR3DL2 recombinant antibody (CSB-RA012365MA1HU), the EC50 is 14.18-23.93 ng/mL. Form: Lyophilized powder. Buffer: Lyophilized from a 0.2 µm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Receptor on natural killer (NK) cells and T cells for MHC class I molecules. Upon binding of peptide-free HLA-F open conformer, negatively regulates NK and T cell effector functions. Acts as a receptor on astrocytes for HLA-F. Through interaction with HLA-F, may protect motor neurons from astrocyte-induced toxicity. Reference: "Cloning of immunoglobulin-superfamily members associated with HLA-C and HLA-B recognition by human natural killer cells." Colonna M., Samaridis J. Science 268:405-408 (1995) "Killer cell inhibitory receptors specific for HLA-C and HLA-B identified by direct binding and by functional transfer." Wagtmann N., Rajagopalan S., Winter C.C., Peruzzi M., Long E.O. Immunity 3:801-809 (1995). Function: nan