Kallikrein 1-related peptidase b5 (Klk1b5), mouse, recombinant

Organism: Mus musculus (Mouse). Source: E.coli. Expression Region: 25-261aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: IFGGFNCEKN SQPWQVAVYR FTKYQCGGVL LNANWVLTAA HCHNDKYQVW LGKNNFFEDE PSAQHRLVSK AIPHPDFNMS LLNEHTPQPE DDYSNDLMLL RLKKPADITD VVKPIDLPTE EPKLGSTCLA SGWGSITPVI YEPADDLQCV NFKLLPNEDC VKAHIEKVTD VMLCAGDMDG GKDTCMGDSG GPLICDGVLH GITSWGPSPC GKPNVPGIYT KLIKFNSWIK DTIAKNA. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Glandular kallikreins cleave Met-Lys and Arg-Ser bonds in kininogen to release Lys-bradykinin. Reference: "Large-scale sequencing of the CD33-related Siglec gene cluster in five mammalian species reveals rapid evolution by multiple mechanisms." Angata T., Margulies E.H., Green E.D., Varki A. Proc. Natl. Acad. Sci. U.S.A. 101:13251-13256(2004). Function: nan