Integrin alpha-X (Itgax), partial, mouse, recombinant

Organism: Mus musculus (Mouse). Source: Baculovirus. Expression Region: 20-1116aa. Protein Length: Partial. Tag Info: N-terminal MBP-tagged and C-terminal 6xHis-tagged. Target Protein Sequence: FNLDAEKLTH FHMDGAEFGH SVLQYDSSWV VVGAPKEIKA TNQIGGLYKC GYHTGNCEPI SLQVPPEAVN ISLGLSLAAA TNPSWLLACG PTVHHTCREN IYLTGLCFLL SSSFKQSQNF PTAQQECPKQ DQDIVFLIDG SGSISSTDFE KMLDFVKAVM SQLQRPSTRF SLMQFSDYFR VHFTFNNFIS TSSPLSLLGS VRQLRGYTYT ASAIKHVITE LFTTQSGARQ DATKVLIVIT DGRKQGDNLS YDSVIPMAEA ASIIRYAIGV GKAFYNEHSK QELKAIASMP SHEYVFSVEN FDALKDIENQ LKEKIFAIEG TETPSSSTFE LEMSQEGFSA VFTPDGPVLG AVGSFSWSGG AFLYPSNMRP TFINMSQENE DMRDAYLGYS TALAFWKGVH SLILGAPRHQ HTGKVVIFTQ ESRHWRPKSE VRGTQIGSYF GASLCSVDMD RDGSTDLVLI GVPHYYEHTR GGQVSVCPMP GVGSRWHCGT TLHGEQGHPW GRFGAALTVL GDVNGDSLAD VAIGAPGEEE NRGAVYIFHG ASRQDIAPSP SQRISASQIP SRIQYFGQSL SGGQDLTRDG LVDLAVGSKG RVLLLRTRPI LRVSPTVHFT PAEISRSVFE CQEQVAPEQT LSDATVCLHI HESPKTQLGD LRSTVTFDLA LDHGRLSTRA IFKETKTRAL TRVKTLGLNK HCESVKLLLP ACVEDSVTPI TLRLNFSLVG VPISSLQNLQ PMLAVDDQTY FTASLPFEKN CGADHICQDD LSVVFGFPDL KTLVVGSDLE LNVDVTVSND GEDSYGTTVT LFYPVGLSFR RVAEGQVFLR KKEDQQWQRR GQHSLHLMCD STPDRSQGLW STSCSSRHVI FRGGSQMTFL VTFDVSPKAE LGDRLLLRAR VGSENNVPGT PKTTFQLELP VKYAVYTMIS SHDQFTKYLN FSTSEKEKTS VVEHRFQVNN LGQRDVPVSI NFWVPIELKG EAVWTVMVSH PQNPLTQCYR NRLKPTQFDL LTHMQKSPVL DCSIADCLHL RCDIPSLGIL DELYFILKGN LSFGWISQTL QKKVLLLSEA EITFNTSVYS QLPGQEAFLR AQTKTVLEMY KVHNPVP. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis. Reference: "A tissue-specific atlas of mouse protein phosphorylation and expression." Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R., Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P. Cell 143:1174-1189(2010). Function: Integrin alpha-X/beta-2 is a receptor for fibrinogen. It recognizes the sequence G-P-R in fibrinogen. It mediates cell-cell interaction during inflammatory responses. It is especially important in monocyte adhesion and chemotaxis (By similarity).