Indoleamine 2,3-dioxygenase 2 (IDO2), human, recombinant

Organism: Homo sapiens (Human). Source: Yeast. Expression Region: 1-407aa. Protein Length: Full length. Tag Info: N-terminal 6xHis-tagged. Target Protein Sequence: MEPHRPNVKT AVPLSLESYH ISEEYGFLLP DSLKELPDHY RPWMEIANKL PQLIDAHQLQ AHVDKMPLLS CQFLKGHREQ RLAHLVLSFL TMGYVWQEGE AQPAEVLPRN LALPFVEVSR NLGLPPILVH SDLVLTNWTK KDPDGFLEIG NLETIISFPG GESLHGFILV TALVEKEAVP GIKALVQATN AILQPNQEAL LQALQRLRLS IQDITKTLGQ MHDYVDPDIF YAGIRIFLSG WKDNPAMPAG LMYEGVSQEP LKYSGGSAAQ STVLHAFDEF LGIRHSKESG DFLYRMRDYM PPSHKAFIED IHSAPSLRDY ILSSGQDHLL TAYNQCVQAL AELRSYHITM VTKYLITAAA KAKHGKPNHL PGPPQALKDR GTGGTAVMSF LKSVRDKTLE SILHPRG. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Catalyzes the first and rate-limiting step in the kynurenine pathway of tryptophan catabolism. Reference: Characterization of an indoleamine 2,3-dioxygenase-like protein found in humans and mice.Ball H.J., Sanchez-Perez A., Weiser S., Austin C.J.D., Astelbauer F., Miu J., McQuillan J.A., Stocker R., Jermiin L.S., Hunt N.H.Gene 396:203-213(2007). Function: Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway