iFluor(R) 790-Wheat Germ Agglutinin (WGA) Conjugate

Correction Factor (260 nm): 0.1. Correction Factor (280 nm): 0.09. Extinction coefficient (cm^-1 M^-1): 250000 (aqueous buffer (pH 7.2)). Excitation (nm): 787. Emission (nm): 812. Quantum yield: 0.13 (aqueous buffer (pH 7.2)). Wheat germ agglutinin (WGA) is a well-studied lectin known for its binding affinity to N-acetyl-D-glucosamine and sialic acid, making it a valuable tool in various biological applications. Its interaction with glycoconjugates enables widespread use of WGA derivatives and conjugates for fluorescence imaging and analysis, facilitating the labeling of yeast bud scars, fibrotic scar tissue, and the cell membranes of gram bacteria and mammalian cells. WGA specifically targets sequences of beta-1,4-GlcNAc-linked residues known as chitodextrins. Each monomer contains two identical, non-interacting binding sites complementary to 3 or 4 beta-1,4-GlcNAc units. Among the monosaccharides tested, only GlcNAc shows strong binding to WGA, while ManNAc demonstrates no binding, and GalNAc exhibits weak binding. The iFluor(R) 790 labeled WGA conjugate exhibits a distinct near-infrared fluorescence characteristic, emitting maximally at 812 nm. Notably, the iFluor(R) 790 WGA conjugate retains its strong affinity for sialic acid and N-acetylglucosaminyl residues, enhancing its utility in fluorescence imaging and analysis for various scientific investigations.