Hemolytic phospholipase C (plcH), partial, Pseudomonas aeruginosa, recombinant

Organism: Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG). Source: E.coli. Expression Region: 51-436aa. Protein Length: Partial. Tag Info: N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged. Target Protein Sequence: VQHVVILMQE NRSFDHYFGH LNGVRGFNDP RALKRQDGKP VWYQNYKYEF SPYHWDTKVT SAQWVSSQNH EWSAFHAIWN QGRNDKWMAV QYPEAMGYFK RGDIPYYYAL ADAFTLCEAY HQSMMGPTNP NRLYHMSGRA APSGDGKDVH IGNDMGDGTI GASGTVDWTT YPERLSAAGV DWRVYQEGGY RSSSLWYLYV DAYWKYRLQE QNNYDCNALA WFRNFKNAPR DSDLWQRAML ARGVDQLRKD VQENTLPQVS WIVAPYCYCE HPWWGPSFGE YYVTRVLEAL TSNPEVWART VFILNYDEGD GFYDHASAPV PPWKDGVGLS TVSTAGEIEV SSGLPIGLGH RVPLIAISPW SKGGKVSAEV FDHTSVLRFL ERRFGV. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Hydrolyzes sphingomyelin in addition to phosphatidylcholine. Reference: "Nucleotide sequence and expression of a phosphate-regulated gene encoding a secreted hemolysin of Pseudomonas aeruginosa." Pritchard A.E., Vasil M.L. J. Bacteriol. 167:291-298(1986). Function: Hydrolyzes sphingomyelin in addition to phosphatidylcholine.