HDAC3, Recombinant, Human (Histone Deacetylase 3)

HDAC3 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC3 probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1, increases YY1 repression activity. It is required to repress transcription of the POU1F1 transcription factor. NCOR2 mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription , HDAC1 (human, recombinant, full length, C-terminal His tag, MW: 49.7kD) and human NCOR2 (aa 395-489 N-terminal GST tag, MW: 39 kD) (GenBank Accession No. NM_006312) co-expressed in baculovirus expression system. Source: Sf9 cells , Application: useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Specific Activity: 1 U/ug , Unit Definition: One U =1 pmol/min, , Assay condition: 25mM TrisCl, pH8.0, 137 mM NaCl, 2.7mM KCl, 1mM MgCl2, 0.1mg/ml BSA, 10uM Biomol substrate, 20ng/ul HDAC1. Incubation condition: 60 min at 30ºC. Storage and Stability: Aliquot to avoid repeated freezing and thawing and freeze at -70°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquots are stable for at least 6 months.