Glutaminase liver isoform, mitochondrial (GLS2), human, recombinant

Organism: Homo sapiens (Human). Source: E.coli. Expression Region: 15-602aa. Protein Length: Full Length of Mature Protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: GSHCGRGGWG HPSRSPLLGG GVRHHLSEAA AQGRETPHSH QPQHQDHDSS ESGMLSRLGD LLFYTIAEGQ ERIPIHKFTT ALKATGLQTS DPRLRDCMSE MHRVVQESSS GGLLDRDLFR KCVSSNIVLL TQAFRKKFVI PDFEEFTGHV DRIFEDVKEL TGGKVAAYIP QLAKSNPDLW GVSLCTVDGQ RHSVGHTKIP FCLQSCVKPL TYAISISTLG TDYVHKFVGK EPSGLRYNKL SLNEEGIPHN PMVNAGAIVV SSLIKMDCNK AEKFDFVLQY LNKMAGNEYM GFSNATFQSE KETGDRNYAI GYYLKEKKCF PKGVDMMAAL DLYFQLCSVE VTCESGSVMA ATLANGGICP ITGESVLSAE AVRNTLSLMH SCGMYDFSGQ FAFHVGLPAK SAVSGAILLV VPNVMGMMCL SPPLDKLGNS HRGTSFCQKL VSLFNFHNYD NLRHCARKLD PRREGAEIRN KTVVNLLFAA YSGDVSALRR FALSAMDMEQ KDYDSRTALH VAAAEGHIEV VKFLIEACKV NPFAKDRWGN IPLDDAVQFN HLEVVKLLQD YQDSYTLSET QAEAAAEALS KENLESMV. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation. Reference: "Molecular cloning, sequencing and expression studies of the human breast cancer cell glutaminase."Gomez-Fabre P.M., Aledo J.C., del Castillo-Olivares A., Alonso F.J., Nunez de Castro I., Campos J.A., Marquez J.Biochem. J. 345:365-375(2000). Function: Plays an important role in the regulation of glutamine catabolism. Promotes mitochondrial respiration and increases ATP generation in cells by catalyzing the synthesis of glutamate and alpha-ketoglutarate. Increases cellular anti-oxidant function via NADH and glutathione production. May play a role in preventing tumor proliferation.