Glutaminase (EC 3.5.1.2), Escherichia coli

Glutaminase (EC 3.5.1.2) is purified from a recombinant E. coli strain. Glutaminase is an amidohydrolase enzyme which generates glutamate from glutamine. Glutaminase has tissue-specific isoenzymes. Importantly, glutaminase is found in glial cells. Glutaminase is expressed in periportal hepatocytes, where it generates NH3 (ammonia) for urea synthesis, as does glutamate dehydrogenase. Glutaminase is also expressed in the epithelial cells of the renal tubules, where the produced ammonia is excreted as ammonium ions. This excretion of ammonium ions is an important mechanism of renal acid-base regulation. During an acidosis, glutaminase is induced in the kidney, which leads to an increase in the amount of ammonium ions excreted. The enzyme is provided in 2.5 M lithium sulphate. Activity: 2500 U/ml Temperature and pH optimum/stability: The optimum pH and temperature are 4.9 and 37°C, respectively. Shipping Conditions: Room Temperature. Storage Conditions: 2 °C to 8 °C. Features: >95% pure (SDS-PAGE). ybaS, b0485, Glutaminase 1