FSP1 (human, recombinant)

Ferroptosis suppressor protein 1 (FSP1), also known as apoptosis-inducing factor mitochondria-associated 2 (AIFM2), is a flavoprotein and NAD(P)H-dependent oxidoreductase that inhibits ferroptosis in a glutathione peroxidase 4- and glutathione-independent manner. It is encoded by AIFM2 in humans and is composed of a short N-terminal hydrophobic region followed by a flavin adenine dinucleotide-dependent oxidoreductase domain. FSP1 localizes to the plasma membrane in a myristoylation-dependent manner, where it reduces coenzyme Q10 (CoQ10, Cay-11506) to CoQ10H2. Reduced CoQ10 functions as a radical-trapping antioxidant and inhibits lipid peroxidation. AIFM2 expression positively correlates with resistance to glutathione peroxidase 4 (GPX4) inhibitors, including (1S,3R)-RSL3 (Cay-19288), ML-210 (Cay-23282), and ML-162 (Cay-20455) in cancer cell lines. Withdrawal of the ferroptosis inhibitor ferrostatin-1 (Cay-17729) reduces tumor growth in an Fsp1/Gpx4 double knockout, but not a Gpx4 single knockout, H460 lung cancer mouse xenograft model, indicating that FSP1 confers resistance to ferroptotic cell death. Cayman's FSP1 (human, recombinant) protein can be used for enzyme assay applications.Synonyms: AIFM2, Apoptosis-inducing Factor Homologous Mitochondrion-associated Inducer of Death, Apoptosis-inducing Factor Mitochondria-associated 2, Ferroptosis Suppressor Protein 1, p53-Responsive Gene 3 Protein, PRG3. Purity: >70% estimated by SDS-PAGE. Source: Active recombinant N-terminal His-tagged human FSP1 expressed in E. coli. Amino Acids: 2-373. MW: 42.5 kDa. Formulation: (Request formulation change), 50 mM potassium phosphate, pH 7.8, with 200 mM sodium chloride, 10% glycerol, and 0.1 mM TCEP. UniProt Accession #: Q9BRQ8.