Formimidoyltransferase-cyclodeaminase (FTCD), partial, Biotinylated, swine, recombinant

Organism: Sus scrofa (Pig). Source: E.coli. Expression Region: 1-326aa. Protein Length: Partial. Tag Info: N-terminal MBP-tagged and C-terminal 6xHis-Avi-tagged. Target Protein Sequence: MSQLVECVPN FSEGKNQEVI DAISRAVAQT PGCVLLDVDS GPSTNRTVYT FVGRPEDVVE GALNAARAAY QLIDMSRHHG EHPRMGALDV CPFIPVRGVT MDECVRCAQA FGQRLAEELG VPVYLYGEAA RTAGRQSLPA LRAGEYEALP EKLKQAEWAP DFGPSAFVPS WGATVAGARK FLLAFNINLL STREQAHRIA LDLREQGRGK DQPGRLKKVQ AIGWYLDEKN LAQVSTNLLD FEVTGLHTVF EETCREAQEL SLPVVGSQLV GLVPLKALLD AAAFYCEKEN LFLLQDEHRI RLVVNRLGLD SLAPFKPKER IIEYLV. Purity: Greater than 85% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool., Binds and promotes bundling of vimentin filaments originating from the Golgi. Reference: "The crystal structure of the formiminotransferase domain of formiminotransferase-cyclodeaminase: implications for substrate channeling in a bifunctional enzyme." Kohls D, Sulea T, Purisima EO, MacKenzie RE, Vrielink A. Structure 8 35-46 (2000). Function: nan