Flagellin (fliC), Escherichia coli, recombinant

Organism: Escherichia coli (strain K12). Source: E.coli. Expression Region: 2-498aa. Protein Length: Full Length of Mature protein. Tag Info: N-terminal 6xHis-SUMO-tagged. Target Protein Sequence: AQVINTNSLS LITQNNINKN QSALSSSIER LSSGLRINSA KDDAAGQAIA NRFTSNIKGL TQAARNANDG ISVAQTTEGA LSEINNNLQR VRELTVQATT GTNSESDLSS IQDEIKSRLD EIDRVSGQTQ FNGVNVLAKN GSMKIQVGAN DNQTITIDLK QIDAKTLGLD GFSVKNNDTV TTSAPVTAFG ATTTNNIKLT GITLSTEAAT DTGGTNPASI EGVYTDNGND YYAKITGGDN DGKYYAVTVA NDGTVTMATG ATANATVTDA NTTKATTITS GGTPVQIDNT AGSATANLGA VSLVKLQDSK GNDTDTYALK DTNGNLYAAD VNETTGAVSV KTITYTDSSG AASSPTAVKL GGDDGKTEVV DIDGKTYDSA DLNGGNLQTG LTAGGEALTA VANGKTTDPL KALDDAIASV DKFRSSLGAV QNRLDSAVTN LNNTTTNLSE AQSRIQDADY ATEVSNMSKA QIIQQAGNSV LAKANQVPQQ VLSLLQG. Purity: Greater than 90% as determined by SDS-PAGE. Endotoxin: Not test. Biological Activity: n/a. Form: Liquid or Lyophilized powder. Buffer: If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20 °C/-80 °C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 °C/-80 °C. The shelf life of lyophilized form is 12 months at -20 °C/-80 °C. Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week. Relevance: Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. Reference: "Benchmarking yeast two-hybrid systems using the interactions of bacterial motility proteins." Rajagopala S.V., Hughes K.T., Uetz P. Proteomics 9:5296-5302(2009). Function: Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella.